PV92 practical Assignment Example | Topics and Well Written Essays - 1750 words

PV92 practical - Assignment Example This would, therefore, mean that the genomic DNA is not protected from degradation by the cellular enzymes released during the isolation protocol of the DNA. Response to Question 2. In order to carry out a PCR, one requires DNA. This means a cell is important since it is the source of the DNA. The cell can also serve as a source for base pairs, enzymes and primers. Response to Question 3. There are various structures that are often broken in order to release the DNA from a cell. These include the cells, the cheek tissues, the phosholids, the nucleus, and proteinase K. this is done so that proteins along with other organically-soluble compounds are removed from chromosomes and cells. PCR Amplication. Response to question 1. It is important to have a primer on either side of the segment of a DNA that is going to be amplified because these form the four basic bases. Uracil exists in RNA, despite being a special case. Primers are vital and essential components of the synthesis. They synt hesize an RNA short piece which is complementary to the strant of the template DNA forming the bonds of hydrogen with it. This provides a DNA polymer with a starting poing which that is required for the synthesis. After the completion of the DNA synthesis, the segment of RNA is removed and a DNA replaces it. Response to Question 2. Taq DNA polymerase is the thermostable DNA polymerase that derived its name from the thermophilic bacterium called Thermus aquaticus. The T.aquaticus is a specific bacterium found in hydrothermal and hot springs vents. Response to Question 3. It is because they are the only four basic bases with uracil being is present in the RNA, although it is a special case. Components of Master mix include water, oug template, the buffer, deoxynucleotides, aum primers, template DNA and ampliTaq polymerase. The buffer is used for keeping the master mix at a suitable PH in order to make sure the reaction of the PCR occurs. Deoxynu-cleotides, in this case help in providi ng the energy and the nucleosides used for synthesizing the DNA. As widely cited, it is vital adding equal amounts of each of the nucleotide (, dTTP, dCTP, dATP and dGTP) onto the master matrix for purposes of preventing the mismatch of bases. The ouM primers are short pieces of the DNA that often bind onto the DNA template to allow the Taq DNA polymerase enzyme to start the incorporation of deoxynucleotides. The AmpliTaq polymerase is used, in this case, to add the deoxynucleotides onto the DNA template. Last but not least, there is presence of oug template that is often amplified by a PCR reaction. Response to Question 4. The DNA PCR amplification takes place in repeated cycles of the three temperature-dependent steps. In the first step, a double-stranded DNA or (dsDNA) template becomes denatured. In the second stage, the oligonucleotide primers become annealed to a single-stranded DNA also called (ssDNA) template. In this case one primer become designed specifically to anneal ont o a certain specific region found on the left side of the strands of the DNA with the other primer being designed specifically to anneal onto a specific region of the complementary DNA strand right side. In the third stage, the DNA polymerase is meant to extend on each primer in 3' to 5' direction, while duplicating the fragment of the DNA between the primers. While

MGMT345 U2 IP Research Paper Example | Topics and Well Written Essays - 500 words

MGMT345 U2 IP - Research Paper Example Introduction of anti-racism laws in the company would help in combating the issue of racial discrimination among employees. The law is against the discrimination of employees and staffs with different ethnic races. In the human resources management department, the idea of hiring the employees from a particular race should be discouraged, and recruitment of staffs from different races encouraged (Young, 2006). Punishing of employees, low payments, lack of employees provident benefits and segregating the employees from a certain race is the most essential matter that the HRM should address instantly. Promoting and facilitating equal valuation of the employees despite their races should be practiced in all company undertaking. Young (2006) argues that there are numerous causes of workplace discrimination among many companies all over the world. Racial discrimination may result from the identity superiority of some of the countries over the others with different races. This may result from the movement of residents from the poor or third world countries to countries with advanced economies. In most cases, such workers end up experiencing racial discrimination. The fight for superiority makes the most of racism cases among the different races all over the world. The abuse of the employees-employer relationship causes racial discrimination among the staffs. Granting leave breaks and workstation assignment may cause discrimination among the employees. The leaders may grant the application to those whom they chose over on the basis of race. Setting a platform where all the workers are granted their request equally should be established to ensure that there is an equal treatment of staffs. This can also be enhanced by introducing of a rotational schedule where employees are scheduled to have off duties, breaks and all the benefits they are entitled to, equally. Due to advancements in the U.S, the cases of racism may be easily